The Galleria Borghese houses a substantial part of the Borghese collection of paintings, sculpture and antiquities, begun by Cardinal Scipione Borghese, the nephew of Pope Paul V (reign 1605–1621). The Villa was built by the architect Flaminio Ponzio, developing sketches by Scipione Borghese himself, who used it as a party villa at the edge of Rome.

Scipione Borghese was an early patron of Bernini and an avid collector of works by Caravaggio, who is well represented in the collection by his Boy with a Basket of Fruit, St. Jerome, Sick Bacchus and others. Other paintings of note include Titian's Sacred and Profane Love, Raphael's Entombment of Christ and works by Peter Paul Rubens and Federico Barocci.

Visitors with pre booked tickets have guaranteed timed entrance.